Polymorphism of Microsatellites Designed from Expressed Sequence Tags of Dicot/Monocot Carbonic Anhydrases in Teak (<I>Tectona grandis</I> L.F.) Genome

Vivek Vaishnav; Shashank Mahesh; Pramod Kumar; S. A. Ansari

doi:10.36808/if/2017/v143i12/120382

Polymorphism of Microsatellites Designed from Expressed Sequence Tags of Dicot/Monocot Carbonic Anhydrases in Teak (Tectona grandis L.F.) Genome

Authors

Vivek Vaishnav Institute of Forest Productivity, NH-23, Lalgutwa, Ranchi, Jharkhand, 835 303
Shashank Mahesh Department of Biotechnology, Directorate of Weed Research, Maharajpur, Jabalpur, Madhya Pradesh
Pramod Kumar Genetics and Plant Propagation Division, Tropical Forest Research Institute, P.O. RFRC, Mandla Road, Jabalpur, Madhya Pradesh
S. A. Ansari Institute of Forest Productivity, NH-23, Lalgutwa, Ranchi, Jharkhand, 835 303

DOI:

https://doi.org/10.36808/if/2017/v143i12/120382

Keywords:

CO² Sink, Photosynthesis Rate, Polymorphism, NCBI, Isozyme Variation, Cross-Amplification.

Abstract

Tectona grandis L. f. is one of the most afforested tree species known for its great timber quality. The species DNA was crossamplified through a set of co-dominant markers to evaluate the polymorphism of carbonic anhydrase (CA) enzyme variant. EST-SSR primers were designed from the genes encoding CA in four model plant species viz., Arabidopsis thaliana (dicot), Nicotiana tabacum (dicot), Zea mays (monocot) and Oryza sativa (monocot). Twelve out of nineteen primers amplified highly polymorphic bands and those all belong to monocotyledon plants (Zea mays and Oryza sativa). It reveals that the genes encoding carbonic anhydrase in Indian teak are close to the CA sequences found in monocotyledon model plants. On the other hand, alleles amplified by C4 model plants markers were found more frequent in teak genome comparing to others.

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