In Vitro Morphogenetic Responses of a Tropical Tree <I>Terminallia arjuna</I> L.

Abstract

Plantlets via somatic embryogenesis have been produced from leaf derived callus of mature Terminallia arjuna L. tree when cultured on Murashige and Skoog's (MS) medium containing 2.3μM- 23.0μM of 2,4-D. However, the formation of white globular embryos was triggered by the withdrawal of 2, 4-D from the nutrient medium after the exposure for a certain period of time. Embryogenesis and further embryo development as well as secondary somatic embryo formation was preceded in the nutrient medium containing 0.11μM to 0.44μM of BAP.