Determination of Sex in Simarouba Glauca Through Molecular Marker for Improved Production
DOI:
https://doi.org/10.36808/if/2011/v137i9/12407Keywords:
Simarouba glauca, Sex Detection, Molecular Marker, RAPD SCAR MarkerAbstract
A total of 250 random oligonucleotide primers were surveyed to identify sex of Simarouba glauca a tree born oilseed crop. Primer OPA-OS900, was found to produce a consistent marker, which differentiated female from both male and andromonoecious sex plants. An identified random amplified polymorphic DNA (RAPD) marker, OPA-OS, linked to male sex in Simarouba was cloned and sequenced. Their end sequences were used to design an allele-specific sequence characterized amplicon region (SCAR) primer SCAR(12f/r), which was found promising in male and andromonoecious sex. The marker designed was amplified at a specific site of 915 bp only in male and andromonoecious plants. This would help to identify the sex of tree born oilseed crop Simarouba.Downloads
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Published
2011-09-01
How to Cite
Prasanthi, L., Bhaskara Reddy, B. V. ., Rekha Rani, K. ., Maheswara Reddy, P. ., Rajeswari, T., Sivaprasad, Y., & Raja Reddy, K. . (2011). Determination of Sex in Simarouba Glauca Through Molecular Marker for Improved Production. Indian Forester, 137(9), 1066–1070. https://doi.org/10.36808/if/2011/v137i9/12407
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