Molecular and Palynological Evidences in Relation to the Hierarchy of <I>Thevetia peruviana</I> Morphovariants

Nesy Ea; Lizzy Mathew

doi:10.36808/if/2020/v146i11/150626

Molecular and Palynological Evidences in Relation to the Hierarchy of Thevetia peruviana Morphovariants

Authors

Nesy Ea KKTM Govt. College, Pullut, Thrissur, Kerala
Lizzy Mathew KKTM Govt. College, Pullut, Thrissur, Kerala

DOI:

https://doi.org/10.36808/if/2020/v146i11/150626

Keywords:

Colpus, Infraspecific, rbcL.

Abstract

Thevetia peruviana, an ornamental medicinal taxon of Apocynaceae, blooms in various colour shades viz yellow, orange and white which are generally considered as morphological replicas of the original parental type. Studies were conducted to expose the similarities and differences between these three infraspecific taxa using two disciplines like palynology and DNA sequencing, in order to place them at suitable hierarchical level. Even though morphologically plants are similar, marked variations were observed in pollen aperture characters, ornamentation and DNA sequences. Striking variations were recorded in the colpus margin as tapering to wavy; and colpus ends as rounded to rectangular. Similarly, surface of pollen showed foveolo-reticulate, dimorphofoveolate and fossulate ornamentation among the studied morphovariants. Gene sequences were compared with the sequence of T. peruviana in the NCBI database, and yellow flowered plant showed 100% DNA similarity, whereas other two variants showed 99% similarity. Based on the study, it is recommended to give varietal and subvarietal status to the morphovariants of Thevetia peruviana by adding functional trinomials at systematic level which in turn would be advantageous for the future revision of the family.

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