Investigation of Phylogenetic Relationships among Some Iranian Rubus L. species
DOI:
https://doi.org/10.36808/if/2021/v147i1/151912Keywords:
Rubus, Phylogeny, ITS, Morphology, Biosystematics.Abstract
Rubus L. is considered as a most complicated genus of Rosaceae that has worldwide distribution. The fruits of some Rubus species are used by human. In current study, authors used morphological and phylogenetic ITS data for taxonomical treatment of 18 Rubus species, which were harvested from northern regions of Iran. Morphological characteristics were subjected for statistical analyses using SPSS and MVSP softwares. For phylogenetic study, DNA was extracted using MBST kit and the results were amplified using ITS primers. Both qualitative and quantitative morphological traits varied among the studied species. Besides, the analysis of variance test revealed significant variations (p ≤ 0.05) for all the quantitative features. The evaluated species were classified into five distinct groups according to morphological characteristics. In ITS phylogenetic cladogram, the evaluated populations were clustered in three distinct clads. Although, species of each clade have some similar morphological traits, the species clustering patterns were not similar in these diagrams. It seems that apomixes, polyploidy and ecological parameters influenced the morphological characteristics and lead to misidentification of Rubus species. This eventually leads to increase in the number of species or synonyms in the genus.References
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