Standardizing Propagation Techniques for Conservation of Inula racemosa Hook. f. in Kashmir Himalayas
DOI:
https://doi.org/10.36808/if/2024/v150i4/169358Keywords:
Nursery, Seed, Rhizome, Conservation, Himalaya.Abstract
The present study was aimed to standardize propagation techniques for conservation of Inula racemosa Hook. f. in Kashmir Himalayas due to its critically endangered status apart from having high market demand. The experiment was conducted in the research field of Faculty of Forestry, Benhama Ganderbal, SKUAST-Kashmir using RBD and the plant material was treated with stratification and rooting hormones. Among the various stratification treatments, moist stratification of 75 days recorded highest germination (79.33%), survival (55.00%), seedling height (30.42 cm), total biomass of seedling (22.30 g/seedling), seedling quality index (1.22), seedling vigor index (24.13) as compared to control (0.01%). Maximum sprouting (95.00%), rooting (91.67%), plant height (201.93 cm), shoot-root ratio (1.26), total biomass of seedling (691.74), seedling quality index (24.09) were reported when rhizomes were treated with IBA@100ppm. The standardization techniques can be used for mass multiplication of this endangered plant to fulfill the market demand and shall provide livelihood to concerned inhabitants. The development of these procedures and techniques will also be helpful in sustainable utilization and conservation of the species apart from making it of commercial use.References
Amare T. and Bhardwaj D.R. (2015). Effect of stratification media and duration on germination and seedling growth of Quercus glauca Thunb. Journal of Tree Sciences, 34(2): 12-21.
Arora R.K., Maheshwari M.L., Chandel K.P. and Gupta R. (1980). Mano (Inula racemosa): little known aromatic plant of Lahaul valley, India. Economic Botany, 34(2): 175–180.
Bano H., Bhat J.I.A., Lone F.A., Noor F., Bhat M.A. and Nazir N. (2019). Effect of Phytohormones and Other Dormancy Breaking Chemicals on Seed Germination of Inula racemosa Hook F. – A Critically Endangered Medicinal Plant of North Western Himalaya. International Journal of Current Microbiology and Applied Sciences, 8(3): 866-876.
Baskin J.M. and Baskin C.C. (2014). Seeds Ecology, Biogeography and Evaluation of Dormancy and Germination. Academic Press, San Diego.
Bharathy P.V., Sonawane P.C. and Sasnu A. (2004). Effect of plant growth regulators, type of cutting and season of rooting of carnation (Dianthus caryophyllus L.) cuttings. Indian Journal of Horticulture, 61: 338-341.
Borah R., Malngiang L., Upadhaya K. and Choudhary H. (2023). Seed dormancy, viability and germination of Illicium griffithii: Athreatened medicinal plant of northeast India. Journal of the Saudi Society of Agricultural Sciences, 22(8): 1-11.
Butola J.S. and Badola H.K. (2007). Vegetative propagation of Angelica glauca and Heracleum candicans. Journal of tropical medicinal plants, 8(1): 85-91.
Dahkaei M.N.P. (2009). Effect of gibberellic acid, temperature and cold moist stratification on seed germination of Danae racemosa. Acta Horticulturae, 813: 445-452.
Dickson A., Leaf A.L. and Hosner J.F. (1960). Quality Apprissal of White Spruce and White Pine Seedling Stock in Nurceries. Forestry Chronicle, 36(1): 10-13.
Donald D.G.M. (1980). Dormancy control in Pinus patula. In: Proceeding of forest nursery and establishment research working group 3rd meeting. Saasveld Forest Research Station. George South Africa, pp. 66-85. (Forest Abstract 1987) 40(6): 25-49.
Firdous Q., Bhat M.F. and Hussain M.M. (2018). Ethnopharmacology, phytochemistry and biological activity of Inula racemosa hook. f: a review. International Journal of Research in Ayurveda and Pharmacy, 9(1): 95-102.
Hangarter R.P. (1997). Gravity, Light and Plant Form. Plant, Cell and Environment, 20(6): 1365-1366.
Harrington C.A. and Kraft J.M. (2004). Cold stratification of pacific madrone seeds. Native Plant Journal, 26: 66-74.
Huang Z., Zhang X., Zheng G. and Gutterman Y. (2003). Influence of light, temperature, salinity and storage on seed germination of Haloxylonammodendron, Journal of Arid Environments, 55(3): 453–464.
Indian Ayurvedic Pharmacopoeia Committee (1978). Ministry of Health and Family planning, Department of Health pp. 7-8.
Jamna J., Balakrishnan R., Ramakrishnan P., Sarala S. and Jolly C.I. (2012). Comparative pharmacognostic study of Inula racemosa Hook. f. and its adulterant Coffea travancorensis Wight & Arn. Indian Journal of Natural Products and Resources, 3(3): 386–394.
Kandari Z.L.S., Roa K.S., Chauhan K., Maikihuri R.K., Purohit V.K., Phondani P.C. and Saxena K.G. (2007). Effect of presowing treatments on seed germination of two endangered medicinal herbs of the Himalaya (Angelica glauca Edgew and Pleurospermum angelicoides wall.EX DC. Benth. Ex C.B Clarke). Proc. Indian Natiomal Science Academy, 73:11-16.
Kazankaya A., Yoruk E. and Dogan A. (2005). Effect of IBA on rooting of Rosa canina hardwood cuttings from Lake Van Region, Turkey. Acta Horticulturae, 690: 153-158.
Khurana P., Singh A. and Saroch V. (2015). Role of Pushkarmool in the asthma management: a conceptual study. Journal of Traditional and Natural Medicine, 1(1): 10–12.
Lokhande P.D., Jawai K.R., Kodam K.M., Kucherkar B.S., Chbukwar A.R. and Jagdale S.C. (2007). Antibacterial activity of isolated constituents and extract of roots of Inula racemosa. Research Journal of Medicinal Plants, I: 7-12.
Mugloo J.A., Mir N.A., Khan P.A., Perray G.N. and Kaisar K.N. (2017). Determination of effect of cold stratification temperature and duration on germination of spruce (Picea smithiana Wall. Boiss) under Laboratory Conditions. Journal of Experimental Agriculture International, 16(1): 1-10.
Nadeem M., Paln L.M.S., Purohit A.N., Pandey H. and Nandi S.K. (2002). Propagation and conservation of Podophyllum hexandrum royle: an important medicinal herb. Biological Conservation, 92: 121-129.
Negi P., Nautiyal B.P., Thakur N., Negi M. and Meena V.K. (2017). Effect of different pre sowing treatments on seed germination and seedling growth of Walnut (Juglans regia L.). International Journal of Current Microbiology and Applied Sciences, 6(7): 3844-3849.
Ojha S., Bharti S., Sharma A.K., Rani N., Bhatia J., Kumari S. and Arya D.S. (2011). Effect of Inula racemosa root extract on cardiac function and oxidative stress against isoproterenol-induced myocardial infarction. Indian Journal of Biochemistry and Biophysics, 48(1): 22–28. PMID: 21469598
Parvin P, Khezri M, Tavasolian I. and Hosseini H. (2015). The effect of gibberellic acid and chilling stratification on seed germination of Eastern black walnut (Juglans nigra L.). Journal of Nuts, 6(1): 67-76.
Peng D.L., Geng B.Y., Qin Y.B., Yang L.E., Baskin C.C. and Baskin J.M. (2023). Ecophysiology of seed dormancy and germination in the alpine-subalpine medicinal plant species Sinopodophyllum hexandrum (Royle) TS Ying. Journal of Applied Research on Medicinal and Aromatic Plants, 32:100448.
Rathore S., Raj, Y., Debnath P., Kumar, M. and Kumar R. (2022). Ethnopharmacology, phytochemistry, agrotechnology, and conservation of Inula racemosa Hook f.–A critically endangered medicinal plant of the western Himalaya. Journal of ethnopharmacology, 283:114613.
Rawat J.M.S., Tomar Y.K. and Rawat V. (2010). Effect of stratification on seed germination and seedling performance of wild pomegranate. Journal of American Science, 6(5): 97-99.
Rawat Y.S. and Everson C.S. (2011). Inula racemosa Hook. f: a potential medicinal crop in the cold desert agro-ecosystem of North Western Himalaya, India. Journal of Medicinal Plants Research, 5(26): 6218–6223. https://doi.org/10.5897/JMPR.9000821.
Rinchen T., Patel M.K., Dolkar P., Stobgais T., Gurmet P., Dawa S., Chosdup T., Angdus T. and Dolma T. (2019). Agro-technique of critically endangered and commercially viable medicinal plant Inula racemosa Hook.f. in cold desert region of Ladakh, India. Journal of medicinal plants studies, 7(4): 47–50.
Satyawarthi G.V., Gupta A.K., Tandon N. and Seth S.D. (1987). Medicinal plants of India. Indian Council of Medicinal Research, New Delhi. Vol. II.
Shabir P.A., Nawchoo I.A. and Wani A.A. (2010). Development of Vegetative and Sexual Multiplication Protocol for Commercialization of Inula racemosa Hook. F. A critically endangered medicinal plant of north Western Himalaya. Nature and Science, 8(10): 246-252.
Sharma R.K. and Sharma S.S. (2006). Seed Germination Behavior of some Medicinal Plants of Lahaul and Spiti cold desert (Himachal Pradesh): Implication for Conservation and Cultivation. Current Science, 90(8): 1113-1118.
Sharma R.K. and Sharma S.S. (2010). Seed physiological aspects of pushkarmool (Inula racemosa), a threatened medicinal herb: response to storage, cold stratification, light and gibberellic acid. Current Science, 99(12): 1801-1806.
Sharma V., Hem K., Sharma D., Singh V.P. and Singh N.K. (2016). Ethnopharmacology, phytochemistry and pharmacology of Inula racemosa Hook. F. Journal of Natural Product Research, 2(1): 40–46.
Sofi P.A., Aslam M., Masoodi T.H., Pala N.A., Rashid M. and Ishtiyak P. (2020). Influence of seed source and stratification on germination and growth of Betula utilis D. Don under temperate conditions in Kashmir Himalaya. Indian Forester, 146(6): 532-537.
Sofi P.A., Rasheid N., Masoodi T.H. and Parray A. (2018). Standardization of pre-sowing treatments for seed germination and seedling growth of Ginkgo biloba L. under temperate conditions of Kashmir Himalayas. Journal of Pharmacognosy and Phytochemistry, 7(2): 1249-1253.
Soni M.K. and Sharma O. (2018). Pushkarmoola (Inula racemosa Hook. F.): an ayurvedic review. World Journal of Pharmaceutical and Medical Research, 4(3): 199–201.
Sun C.P., Jia Z.L., Huo X.K., Tian X.G., Feng L., Wang C., Zhang B.J., Zhao W.Y. and Ma X.C. (2021). Medicinal Inula species: phytochemistry, biosynthesis, and bioactivities. American Journal of Chinese Medicine, 49(2): 315–358. https://doi.org/10.1142/ S0192415X21500166.
Tilki F. and Cicek E. (2005). Effects of stratification, temperature and storage on germination in three provenances of Fraxinus angustifolia subsp. Oxycarpa seeds. Turkish Journal of Agriculture, 29: 323-330.
Tripathi Y.B. and Chaturvedi P. (1995). Assessment of Endocrine Response of Inula racemosa in relation to glucose homeostasis in rats. Indian Journal of Experimental Biology, 33: 686-689.
Vashitah R.K., Nautiyal B.P. and Nautiyal M.C. (2009). Presowing treatments to improve seed germination in Angelica glauca Edgew, an endangered medicinal herb of Western Himalayas. Journal of Herbs, Spices and Medicinal Plants, 15(1): 73-85.
Wang J.H., Baskin C.C. and Du W.C.G.H. (2010). Variation in seed germination between populations of five sub-alpine woody species from eastern Qinghai-Tibet Plateau following dry storage atlowtemperatures.EcologicalResearch,25:195-203.
Wang K., Liu H., Zhao Y., Chen X., Hu Z., Song Y. and Ma X. (2000). Separation and determination of Alantolactone and Isoalantolactone in traditional chenese herbs by capillary electophorasis. Talanta, 52: 1001-1005.
Wani P.A., Nawchoo I.A. and Wafai B.A. (2007). Improvement of Sexual Destination in Atropa acuminate Royle (Solanaceae). A Critically Endangered Medicinal Plant of Northwestern Himalaya. Pakistan Journal of Biological Sciences, 10(5): 778-782.
Yang Q.H., Wei X. and Zeng X.L. (2008). Seed biology and germination ecophysiology of Camellia nitidissima. Forest Ecology and Management, 255(1): 113–118.
Young J.A. and Young C.G. (1992). Seeds of woody plants in North America. Portland (OR): Dioscorides Press pp. 407.
Zhao Y.M., Zhang M.L., Shi Q.W. and Kiyota H. (2006). Chemical Constituents of Plants from genus Inula. Journal of chemical Biodiversity, 3: 371-384.
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