Evaluation of Four Different Methods of Genomic DNA Extraction from Quercus leucotricophora

Sneha Dobhal; H. S. Ginwal

doi:10.36808/if/2014/v140i2/46849

Evaluation of Four Different Methods of Genomic DNA Extraction from Quercus leucotricophora

Authors

Sneha Dobhal Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195
H. S. Ginwal Division of Genetics and Tree Propogation, Forest Research Institute University, Dehradun-243195

DOI:

https://doi.org/10.36808/if/2014/v140i2/46849

Keywords:

DNA, Extraction, Quercus leucotricophora.

Abstract

The most essential principal in the modern molecular biology is extraction of DNA with a desirable quantity and quality and this achievement could be the main necessity for every genetic study. DNA extraction is obviously difficult because of negative effects obtained from carbohydrates, tannins, polyphenols and proteins. Extraction of DNA from Quercus leucotricophora, was conducted by four different methods. The most value of qualified DNA extracted from young leaf tissue was 629.9 to 1412.1 ng/Î¼l by the method applied by Doyle and Doyle and Stange and his associates with some modifications. Accordingly above method is recommended for DNA extraction from young leaves of Quercus leucotricophora.

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