Adventitious Shoot Regeneration and <I>Agrobacterium tumefaciens</I> Mediated Transformation in Rohida (<I>Tecomella undulata</I>)

Abstract

In the present investigation four different explants from Tecomella undulata were used for in vitro adventitious shoot regeneration. The treatment having IAA (0.1 mg/l) and zeatin (2.5mg/l) in combination yielded maximum number of shoots from hypocotyle (16) and cotyledonary nodes (27). In case of epicotyle, the best response (11 shoots per explant) was observed in the treatments having IAA (0.1 mg/l) + BAP (2.5 mg/l). While, regeneration of shoots from cotyledon was not observed. Pretreatment of regenerated shoots in a mixture of NAA, IAA and IBA (5.0 mg/l, each) for 36 hours was found to be the best for root induction. Survival of 75% was observed from the plantlets kept for hardening, whereas 62% hardened plants survived in field. For transformation, continuous light and mild shaking with hands at three to five hours interval for 36 hours was found more efficient (5% transformation). Hence, we report here an efficient adventitious shoot regeneration system in T. undulata using cotyledonary node as an explant that has a vital role in gene transfer technologies.