Evaluation of Genetic Diversity in Bamboo Species through SDS-Page Protein Analysis

M. S. Bhandari; R. Kaushal; R. L. Banik; S. K. Tewari

doi:10.36808/if/2015/v141i8/76991

Evaluation of Genetic Diversity in Bamboo Species through SDS-Page Protein Analysis

Authors

M. S. Bhandari Division of Genetics & Tree Propagation, Forest Research Institute, Dehradun (Uttarakhand)
R. Kaushal Division of Plant Science, Central Soil and Water Conservation and Research and Training Institute, Dehradun-248 195
R. L. Banik Department of Genetics and Plant Breeding, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145
S. K. Tewari Department of Genetics and Plant Breeding, G.B. Pant University of Agriculture and Technology, Pantnagar-263 145

DOI:

https://doi.org/10.36808/if/2015/v141i8/76991

Keywords:

Bamboo, Genetic Diversity, Electrophoretic Pattern, Upgma and Jaccard's Similarity Coefficient.

Abstract

Bamboos serve as multipurpose forest grass species. The success of bamboo as a commercially important forage species depends on the identification of genetically divergent materials of the plant and developing superior planting stock for the farmers. The phylogeny plays a crucial role in the evolution of species and superior germplasm. Twentytwo species of bamboo were evaluated for genetic diversity characterization through SDS-PAGE analysis. Based on electrophoretic pattern, banding pattern was established for 22 species. Also, on genetic diversity analysis, 22 species of bamboo were grouped into four clusters. Cluster IIBb had maximum number of species (9), cluster IIBa and cluster IIAb had four species each, whereas, Cluster IIAa and cluster IA had maximum of two and three species respectively. UPGMA (Unweighed Paired Group Mean Cluster Analysis) inferred that among Bambusa genera; species B. bambusa, B. multiplex, B. vulgaris, B. balcooa, B. tulda, B. nutans, B. polymorpha, B. nutans and B. pallida and among Dendrocalamus species D. hamiltonii, D. giganteus, D. membraneceous and D. longispathus were genetically similar having Jaccard's similarity coefficient ranged from 0.80-1.00.

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