Variation among Genotypes of Tectona grandis L.f for in-vitro Axillary Shoot Multiplication and Adventitious Rhizogenesis
DOI:
https://doi.org/10.36808/if/2016/v142i11/105602Keywords:
Adventitious Rhizogenesis, Axillary, Genotype, In-vitro, Tectona grandis, Variation.Abstract
The influence of genotype at the family level on axillary shoot multiplication and adventitious rhizogenesis in Tectona grandis was examined using in vitro raised seedlings. Three half-sib families were investigated for their ability to produce axillary shoots and in vitro roots. Shoot multiplication and rooting was obtained in all families, though efficiency varied depending on genotype and concentrations of plant growth regulators. Three way factorial experiment was conducted with three families, 5 levels of BA (6-benzyl adenine) and 5 levels of NAA (1-naphthalene acetic acid). Genotypic variation for axillary shoot multiplication was observed among the three half-sib families, with a significant interaction between family, BA and NAA concentrations. The combination of C-45 family, 10 μM BA and 1 μM NAA was found to be the most effective for axillary shoot multiplication. A range of auxins (NAA, IBA- indole 3-butyric acid, IAA - indole 3-acetic acid, IPAindole 3-propionic acid) in three concentrations each (10, 15 and 20 μM) were tested for rooting. Efficacy of NAA was the highest for rooting percentage, root number and root length. This study demonstrates the presence of variation among various half-sib families of Tectona grandis for axillary shoot production and adventitious rhizogenesis and it can be utilized for maximizing in vitro production of elite planting material by ameliorating plant growth regulator requirement in the medium.References
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