Improvement in Propagation through Seed Pre-Treatment in Medicinally Important Plant Cowitch (<i>Mucuna pruriens</i> (L.) DC.)

Vikas Yadav Patade; Nirbhay Singh; Atul Grover; Madhu Bala

doi:10.36808/if/2025/v151i1/166653

Improvement in Propagation through Seed Pre-Treatment in Medicinally Important Plant Cowitch (Mucuna pruriens (L.) DC.)

Authors

Vikas Yadav Patade Defence Institute of Bio-Energy Research, Haldwani 263139, Nainital, Uttarakhand
Nirbhay Singh Defence Institute of Bio-Energy Research, Haldwani 263139, Nainital, Uttarakhand
Atul Grover Defence Institute of Bio-Energy Research, Haldwani 263139, Nainital, Uttarakhand
Madhu Bala Defence Institute of Bio-Energy Research, Haldwani 263139, Nainital, Uttarakhand

DOI:

https://doi.org/10.36808/if/2025/v151i1/166653

Keywords:

Cowitch, Seed, Germination, Pre-Treatment, Acid Scarification.

Abstract

Cowitch (Mucuna pruriens (L.) DC.) is an important medicinal plant. Germination was found very poor and slow in a genotype of the seed propagated plant. Present study reports effect of scarification with concentrated (98% v/v) sulfuric acid for different durations (5, 10, 30, 60, 120 min) followed by water soaking for 24 h on seed germination and seedling establishment. Acid pre-treatment for 120 min resulted in significantly (P â‰¤ 0.05) higher seed germination (95.0%) than the control (27.5%), water soaked (27.5%), and the shorter acid treatments. Further, the rate of germination was significantly higher in the acid pre-treatment for 120 min (Timson's Index: 76.0) than the control (Timson's Index: 14.0) and the shorter acid treatments. The seedling establishment was also significantly improved in the acid treatment (92.0%) than the control (20.5%). The acid pre-treatment to the seeds did not cause any adverse effects on seedling vigor, morphology and reproductive growth of the plants. The present study thus suggests acid treatment for 120 min for higher and rapid seed germination for propagation of the medicinally important plant.

References

Aeron A., Maheshwari D.K., Dheeman S., Agarwal M., Dubey R.C., Bajpai V.K. (2017). Plant growth promotion and suppression of charcoal-rot fungus (Macrophomina phaseolina) in velvet bean (Mucuna pruriens L.) by root nodule bacteria. Journal of Phytopathology, 165: 463-478. DOI: https://doi.org/10.1111/jph.12581

Gurumoorthi P., Pugalenthi M. and Janardhanan K. (2003a). Nutritional potential of five accessions of a south Indian tribal pulse Mucuna pruriens var. utilis; II Investigation on total free phenolics, tannins, trypsin and chymotrypsin inhibitors, phytohaemagglutinins, and in vitro protein digestibility. Trop Subtrop Agroecosys, 1:153â€“158

Gurumoorthi P., Senthil K.S., Vadivel V. and Janardhanan K.(2003b). Studies on agrobotanical characters of different accessions of velvet bean collected from Western Ghats, South India. Tropical and Subtropical Agroecosystems, 2: 105-115

Harvais G. and Hadley G. (1967). The development of Orchis purpurella in asymbiotic and inoculated cultures. New Phytologist, 66:217-230 DOI: https://doi.org/10.1111/j.1469-8137.1967.tb06000.x

Kavitha C. and Thangamani C. (2014). Amazing bean Mucuna pruriens: A comprehensive review. J Med Plants Res, 8: 138-143 DOI: https://doi.org/10.5897/JMPR2013.5036

Kelly K.M. and Staden J.V. (1985). Effect of acid scarification on seed coat structure, germination and seedling vigor of Aspalathus linearis. J. Plant Physiol, 121: 37-45 DOI: https://doi.org/10.1016/S0176-1617(85)80089-4

Lampariello L.R., Cortelazzo A., Guerranti R., Sticozzi C. and Valacchi G.(2012). The magic velvet bean of Mucuna pruriens. J. Tradit Complement Med,2: 331â€“339 DOI: https://doi.org/10.1016/S2225-4110(16)30119-5

Misra L. and Wagner H.(2007). Extraction of bioactive principles from Mucuna pruriens seeds. Ind. J. Biochem Biophys,44: 56â€“60

Opoku J.A., Amissah J.N., Essilfie M.E. and Norman J.C. (2018). Effect of pre-sowing treatments on seed germination and seedling growth of silver butterfly tree (Bauhinia rufescens). Curr Agri Res J,6: 344-354 DOI: https://doi.org/10.12944/CARJ.6.3.13

Patade V.Y., Kumar K., Gupta A.K., Grover A., Negi P.S. and Dwivedi S.K. (2019). Augmentation in seed germination through pre-treatments in Himalayan Yellow Raspberry (Rubus ellipticus Sm.): An important wild edible ethno-medicinal shrub of Himalaya. Indian Forester, 145 (4): 339-342

Qadir M. and Khan F. (2020). Effect of various pre-treatments for breaking the dormancy of Mucuna pruriens Bak. Indian J Applied & Pure Bio. 35: 127-132

Thakur I., Kumar J., Routray A., Bakhla R., Kumari J., Runda A. and Dhanyashri P.V.(2018). Screening of superior germplasm of Mucuna pruriens (L.) based upon germination parameters. J Pharmacognosy Phytochem SP, 1: 1952-1956

Wanjekeche E., Wakasa V. and Mureithi J.G. (2003). Effect of germination, alkaline and acid soaking and boiling on the nutritional value of mature and immature Mucuna (Mucuna pruriens) beans. Trop Subtrop Agroecosys, 1:183-192