Assessment of Genetic Variation among Three Different Flower Colour Morphotypes of Tecomella undulata Using Start Codon Targeted Markers
DOI:
https://doi.org/10.36808/if/2025/v151i4/170455Keywords:
Conservation, Genetic Diversity, Morphotypes, Rohida, Start codon targeted markers.Abstract
Tecomella undulata commonly known as Rohida is an important timberyielding tree species of arid and semi-arid regions. It has become victim of overexploitation for its high quality timber and medicinal values because of which the species is categorized under threatened plants. Three different flower colour morphotypes viz., yellow, red and orange were recorded in the species. Flowers of Rohida are recognised as state flower of Rajasthan. To investigate the variation exists among these three different flower colour morphotypes of Rohida, Start Codon Targeted Polymorphism marker system was used. In the present study, 63 SCoT primers were tested, out of which 14 primers were found to be polymorphic generating 1045 scorable bands. Scorable bands per primer were obtained in the range of 20-118. The PIC and RP values of the primers ranged between 0.27-0.45 and 2.80-16.86. The estimated Jaccard similarity coefficient ranged from 0.038 to 0.579. The dendrogram constructed using Jaccard's coefficient resulted into two clusters, the first cluster including yellow morphotypes and the second cluster including red and orange flower colour morphotypes.
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