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Conservation of Ceropegia maccannii Ansari through in vitro Raised Seedlings

Affiliations

  • Agharkar Research Institute, G.G. Agarkar Road, Pune, (Maharashtra), Iran, Islamic Republic of
  • Agharkar Research Institute, G.G. Agarkar Road, Pune, (Maharashtra), India

Abstract


A protocol is described for micropropagation of critically endangered Ceropegia maccannii Ansari (Asclepiadaceae) having limited reproducing capacity in nature, through in vitro raised seedlings. The effect of cytokinins; 6-benzylaminopurine (BAP), 6-furfurylaminopurine/Kinetin (Kin), thidiazuron (TDZ) and auxins; indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and á-naphthalene acetic acid (NAA) at various concentrations on in vitro regeneration were investigated. The highest number of nodes per explant (8.00±0.31) and maximum shoot length (9.16±0.35 cm) were found on full strength MS medium supplemented with BAP (11.10 μM). Whereas, IBA (12.30 μM) showed maximum root length (2.85±0.18 cm) and number of roots per shoot (6.62±0.53) with maximum shoot length (8.87±0.63 cm). The developed method can be successfully employed for large-scale multiplication and conservation of Ceropegia maccannii Ansari.

Keywords

Ceropegia maccannii, Critically Endangered, Micropropagation, Reintroduction.

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