<I>In vitro</I> Seed Germination Studies of <I>Drypetes roxburghii</I> (Wall) Hurusava

K. Sri Rama Murthy; M. Chandrasekhara Reddy

doi:10.36808/if/2016/v142i2/88786

In vitro Seed Germination Studies of Drypetes roxburghii (Wall) Hurusava

Authors

K. Sri Rama Murthy School of Conservation Biology and Plant Biotechnology, Department of Botany and Biotechnology, Montessori Mahila Kalasala, Vijayawada, Andhra Pradesh
M. Chandrasekhara Reddy School of Conservation Biology and Plant Biotechnology, Department of Botany and Biotechnology, Montessori Mahila Kalasala, Vijayawada, Andhra Pradesh

DOI:

https://doi.org/10.36808/if/2016/v142i2/88786

Keywords:

Seed Germination, Viability, D. roxburghii.

Abstract

This study was designed to analyse seed germination barriers and effect of storage duration on the seed viability of Drypetes roxburghii. Present study showed that the hard seed coat was affecting the in vivo seed germination. The decoated seeds showed 83.3% of germination under in vitro conditions on optimum medium of MS + 0.5 mg/l GA3 + 0.01% AC. The seeds of D. roxburghii showed maximum seed viability at storage period of 7 days and declines with further increase of the storage period.

References

Ahmed M. and Gogoi P. (2000). Agar: History and scope of plantation a perspective. All Assam agar traders and agar oil manufacturersâ€™ association, Nagaon, Assam, India.

Badole M., Dighe V. and Charegaonkar G. (2011). Simultaneous quantification of Ã‚-amyrin and stigmasterol in Putranjiva roxburghii Wall. by High-performance Thin-Layer Chromatography, International journal of Pharma and Bio Sciences, 2:346-352.

Bag N., Chandra S., Palni L.M.S. and Nandi S.K. (2000). Micropropagation of Devringal [Thamnocalamus spathiflorus (Trin.) Munro]-a temperate bamboo, and comparison between in vitro propagated plants and seedlings, Plant Science, 156:125-135.

Bowes B.G. (1999). A Colour Atlas of Plant Propagation and Conservation. Manson Publishing Ltd, London.

Cerabolini B., Andreis R.D., Ceriani R.M., Pierce S. and Raimondi B. (2004). Seed germination and conservation of endangered species from the Italian Alps: Physoplexis comosaand Primula glaucescens, Biol Conserv., 117:351â€“356.

Chaturani G.D.G., Subasinghe S. and Jayatilleke M.P. (2006). In-vitro establishment, germination and growth performance of Red Sandal wood (Pterocarpus santalinus L.), Tropical Agricultural Research & Extension 9:116-130.

Garg H.S. and Mitra C.R. (1968). Putranjiva roxburghiiWall.-II: Triterpenes of the trunk bark, Phytochemistry, 7: 2053-2055.

Garg H.S. and Mitra C.R. (1971). Putraflavone, a new biflavonoid from Putranjiva roxburghii, Phytochemistry, 10: 2787-2791.

Ishii K. and Kambou S. (2007). In vitro culture of an African multipurpose tree species: Adansonia digitata L, Propagation of Ornamental Plants, 7:62-67.

Maridass N. and Thangavel K. (2008). Conservation Efforts Through In Vitro Seed Germination of a Hepatoprotective Plant - Phyllanthus beddomie (Gamble) Mohanan, Ethnobotanical Leaflets, 12:419-424.

Miller A.R., Scheerens J.C., Erb P.S. and Chandler C.K. (1992). Enhanced strawberry seed germination through in vitro culture of cut achenes, J. Amer. Soc. Hort. Sci., 117:313-316.

Mollik M.A.H., Hassan A.I., Paul T.K., Sintaha M., Khaleque H.N., Noor F.A., Nahar A., Seraj S., Jahan R., Chowdhury M.H. and Rahmatullah M. (2010). A survey of medicinal plant usage by folk medicinal practitioners in two villages by the Rupsha river in Bagerhat District, Bangladesh, American-Eurasian Journal of Sustainable Agriculture, 4:349-356.

Murashige T. and Skoog T. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15:473-497.

Pence V. (1999). The application of biotechnology for the conservation of endangered plants. In: Plant Conservation Biotechnology, (Benson, E.E. Edt.) Chapter 15. Taylor and Francis, London, pp. 227-241.

Pickens K.A., Affolter J.M., Wetzstein H.Y. and Wolf J.H.D. (2003). Enhanced seed germination and seedling growth of Tillandsia eizii in vitro, Hort. Science, 18:101-104.

Rambabu M., Ujjwala D., Ugandar T., Praveen M., Upender M. and Swamy N.R. (2006). Effect of GA3 on enhancement of in vitro seed germination on Gevotia rottleriformis (Euphorbiaceae)-an endangered forest tree, Indian Forester, 131 (1) : 25-30.

Rambabu M., Upender M., Ujjwala D., Ugandhar T., Praveen M. and Swamy N.R. (2005). In vitro zygotic embryo culture of an endangered forest tree Givotia rottleriformisand factors affecting its germination and seedling growth, In Vitro Cellular & Developmental Biologyâ€“Plant, 42:418-421.

Reanmongkol W., Noppapan T. and Subhadhirasakul S. (2009). Antinociceptive, antipyretic, and anti-inflammatory activities of Putranjiva roxburghiiWall. leaf extract in experimental animals, Journal of Natural Medicines, 63:290-296.

Reddy R.A., Mastan M., Naidu C. and Rao P.S. (2001). Seasonal variation in rooting response of stem cuttings of Givotia rottleriformis Grift, Ind. J. Forestry, 24:475-477.

Sahni K.C. (2009). The book of Indian trees, Himalaya publishing house, Second edition 285-289.

Sambe M.A.N., Sagna M. and Sy M.O. (2010). Seed germination and in vitro plant regeneration of Parkia biglobosa (Jacq.) Benth, Afr. J. Biotechnol, 9:3099-3108.

Samuel K., Debashish D., Madhumita B., Padmaja G., Prasad S.R., Murthy V.B.R. and Rao P.S. (2009). In vitro germination and micropropagation of Givotia rottleriformis Griff, In Vitro Cellular & Developmental Biologyâ€“Plant, 5:466-473.

Santapau H. (1966). Common Trees, National Book Trust, India.

Sengupta P. and Mukherjee J. (1968). Terpenoids and related compounds-XI: The structure of roxburgholone, a new triterpenoid constituent of Putranjiva roxburghii, Tetrahedron, 24:6259-6264.

Sengupta P., Ghosh K.S. and Das S. (1997).Chemistry of the constituents of Putranjiva roxburghii, J. Indian Chem. Soc., 74:827-830.

Seth A. (2005). The herbs in Ayurveda, (1^st ed)Vol. IV, Alankar Publishers, Bhavanagar, Gujarat. 4:962-963.

Shahwar D., Raza M.A., Saeed A., Riasat M., Chattha H., Javaid M., Ullah S. and Ullah S. (2012). Antioxidant potential of the extracts of Putranjiva roxburghii, Conyza bonariensis, Woodfordia fruiticosa and Senecio chrysanthemoids, Afr. J. Biotechnol, 11:4288-4295.

Shankar U. (2012). Effect of seed abortion and seed storage on germination and seedling growth in Aquilaria malaccensis Lamk. (Thymelaeaceae), Current Science, 102:596-604.

Singh K.P., Kumar V., Tiwari K.R., Sharma A., Rao C.V. and Singh R.H. (2010). Medico-ethnobotany of â€˜Chataraâ€™ block of district Sonebhadra, Uttar Pradesh, India, Advances in BiologicalResearch, 4:65-80.

Thokozani B.L.K., Zulu D., Sileshi G.W., Teklehaimanot Z., Gondwe D.S.B., Sarasan V. and Stevenson P. (2011). Seed germination and In vitro regeneration of the African medicinal and pesticidal plant, Bobgunnia madagascariensis, Afr. J. Biotechnol, 10:5959-5966.

Vaghasiya Y. and Chanda S. (2009). Screening of some traditionally used Indian plants for antibacterial activity against Klebsiella pneumonia, Journal of Herbal Medicine and Toxicology, 3:161-164.

Varma A., Jain S.K. and Alok S. (2010). Hypoglyceic activity of Putranjiva roxburghii Wall. in alloxan induced diabetic rats, International Journal of Pharmaceutical Sciencesand Research, 1:174-178.

Yang J., Lovett-Doust J. and Lovett-Doust L. (1999). Seed germination patterns in green dragon (Arisaema dracontium, Araceae), American Journal of Botany, 86:1160-1167.