Histological Investigations During <i>In-Vitro</i> Adventitious Shoot Regeneration in Leaflet Explants from Nodal Segments of a Mature Tree of <i>Albizia procera</i> Benth.

Ekta Rai; Shamim Akhtar Ansari

doi:10.36808/if/2019/v145i2/144262

Histological Investigations During In-Vitro Adventitious Shoot Regeneration in Leaflet Explants from Nodal Segments of a Mature Tree of Albizia procera Benth.

Authors

Ekta Rai Genetics and Plant Propagation Division Tropical Forest Research Institute, Jabalpur-482021
Shamim Akhtar Ansari Institute of Forest Productivity, Lalgutwa, Ranchi 835303

DOI:

https://doi.org/10.36808/if/2019/v145i2/144262

Keywords:

Leaf Explants, Callus, Adventitious Shoot Differentiation, Histological Studies.

Abstract

This is a 60 day study of transformation from leaf to callus and adventitious shoot regeneration. For initial 30 days leaf explants were kept on MS medium supplemented with 3% sucrose, 2.5 Î¼M 2,4-D and followed by a subsequent adventitious shoot differentiation phase for another 30 days on half MS medium supplemented with 0.25 Î¼M each of BA and IBA. The histological investigation revealed sequential array of events starting from initiation of callus on upper and lower epidermis of inoculated leaflet to initiation of green shoots from the mass of callus in the culture medium.

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