A Simple Method for The Light Microscopic Wood Anatomical Study Of Indian Tropical Trees, Without Using Microtome
DOI:
https://doi.org/10.36808/if/2023/v149i1/167613Keywords:
Harpullia arborea, Nephelium lappaceum, Sapindus trifoliatus, Secondary Xylem Data.Abstract
The most accepted method for preparing the wood anatomical data is the microtome sections using woodsampies from living orxyiarium specimens. Whereas the sectioning process of these wood samples depends on the availability of good quality microtome and in the case of the xylarium samples, the age and source of samples are often found to be missing and the softening procedures for the dry specimens remain challenging. Based on two strategies, here authors undertook the possibility of a simplified methodology for the wood anatomical study of tropical trees, using fresh samples without depending on a microtome. One was that stem samples were sufficient to measure the qualitative wood anatomical details since It Is tomorrow's heartwood, except color, size, and cell content, everything else is the same as found in the heartwood of a large stem. So, hand-made sections of fresh stem samples were selected and following with simple methodology of boiling and bleaching, the qualitative anatomical characters were analyzed. The second was to measure the quantitative wood anatomical data from the macerated sapwood samples. The wood anatomical study by opting the method of collecting the qualitative data from stem sections and quantitatwe measurements from sapwood samples of three tropical tree species - Harpullia arborea (Blanco) Radik., Nephelium lappaceum L. and Sapindus trifoliatus L. was found to be comparable with the existing wood anatomical details of the taxa.References
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