A Protocol for Recovery of whole Plants through in Vitro Shoots Regeneration from Leaflet Explants of Mature Trees of Albizia procera (Roxb.) Benth
DOI:
https://doi.org/10.36808/if/2017/v143i5/114976Keywords:
Albizia procera, 2, 4-D, Reaflet Explant, BAP, IBA, Shoot Bud Regeneration, Root Initiation.Abstract
The in vitro method has, for the first time, been developed to facilitate shoot formation and recovery of hardened plantlets from leaves of adult trees of A. procera in a cycle of 150 days. Leaves were dissected from micro propagated shoot cultures of mature trees of Albizia procera. Effect of five culture media viz., MS, B5, NN, SH and WPM medium with various concentrations of 2, 4-D were tested to determine the conditions most conducive for callus induction. The highest number of adventitious buds at 30 days after inoculation was obtained on MS medium supplemented with 2.5μM 2, 4-D. The regenerated shoot buds were elongated on half strength MS medium containing 0.25μM each of BAP and IBA, which took 30 days after inoculation. The adventitious shoots obtained so were the in-vitro rooted at 30 days after inoculation on B5 medium supplemented with 3μM IBA. The regenerated plantlets were hardened on sterilized sand.References
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