Standardization of Sterilization Protocol for Nodal and Leaf Explants of Field Grown Mature Tree of <I>Diploknema butyracea</I>

Arti Kunwar; Ajay Thakur

doi:10.36808/if/2017/v143i12/120391

Standardization of Sterilization Protocol for Nodal and Leaf Explants of Field Grown Mature Tree of Diploknema butyracea

Authors

Arti Kunwar Tissue Culture Discipline, Genetics and Tree Propagation Division, Forest Research Institute, Dehradun, Uttarakhand
Ajay Thakur Tissue Culture Discipline, Genetics and Tree Propagation Division, Forest Research Institute, Dehradun, Uttarakhand

DOI:

https://doi.org/10.36808/if/2017/v143i12/120391

Keywords:

Tree Borne Oilseeds, Pre-Sterilization, Sterilizing Agent.

Abstract

Standardization of sterilization is the most important step to establish aseptic culture. Experiments were carried out for disinfection of two types of explants; nodal and leaf, taken from mature tree of Diploknema butyracea. Explants were presterilized with an aqueous solution of bavistin (Carbendazim) 0.1-2.0 % for 3-15 minutes. Among all the sterilizing agents viz; HgCl₂, NaOCl and H₂O₂, preeminent result for nodal explant was obtained in 0.2 % HgCl₂ for 15 minutes and for leaf explants treatment with 0.1 % HgCl for 5 minutes was found to be the best.

References

Anand A., Rao C.S. and Balakrishna P. (1999). In vitro propagation of Syzygium travancoricum Gamble â€“ an endangered tree species, Plant Cell, Tissue and Organ Culture, 56: 59-63.

Chauhan J.M.S., Bisht P., Panwar M. and Thakur A. (2015) In vitro propagation of Elaeocarpus sphaericus, Indian Forester, 141 (2): 173-177.

Daud N.H., Jayaraman S. and Mohamed R. (2012). An improved surface sterilization technique for introducing leaf, nodal and seed explants of Aquilaria malaccensis from field sources into tissue culture, Asia Pacific J. Molecular Biology and Biotechnology, 20 (2): 55-58.

He M.L., Qi S.Y. and Hu L.J. (2005). Rapid in vitro propagation of medicinally important Aquilaria agallocha, J. Zhejiang University Science, 6(8): 849-852.

Husain M.K. and Anis M. (2009). Rapid in vitro multiplication of Melia azedarach L. (a multipurpose woody tree), Acta Physiologiae Plantarum, 31: 765-772.

Hussain A., Qarshi I.A., Nazir H. and Ullah I. (2012). Plant tissue culture, Current status and opportunities, Recent Advances in Plant in vitro Culture; Licensee InTech, Open science/open mind, http:// creativecommons.org/licenses/by/3.0.

Omamor I.B., Asemota A.O., Eke C.R. and Ezia E.I. (2007). Fungal contaminants of the oil palm tissue culture in Nigerian Institute for Oil Palm Research (NIFOR), African J. Biotechnology, 2(10): 534-537.

Rather M.M., Thakur A., Panwar M. and Sharma S.K. (2016). In vitro sterilization protocol for icropropagation of chimonobambusa jaunsarensis (gamble) bahadur and naithani- a rare and endangered hill bamboo, Indian Forester, 142 (9): 871-874.

Sharma G. and Nautiyal A.R. (2009). Influence of explants type and plant growth regulators on in vitro multiple shoots regeneration of a Laurel (Cinnamomum tamala Nees. & Ebrm.) from Himalaya, Nature and Science, 7(9): 1-7.

Shu Y.Q., Meng L.H., Li D.L., Chuan H.Z., Lan J.H. and Hui Z.Z. (2005). Production of 2-(2-phenylethyl) chromones in cell suspension cultures of Aquilaria sinensis, Plant Cell, Tissue and Organ Culture, 83: 217-221.

Shukla S., Shukla S.K. and Mishra S.K. (2012). In vitro Regeneration of multipurpose medicinal tree Stereospermum Suaveolensâ€“Factors controlling the in vitro regeneration, J. Biotechnology and Biomaterials, S13:001, (Special Issue 13).

Singh C.K., Sandeep R.R., Patil V.R., Jaiswal P.S. and Subhash N. (2013). Plant regeneration from leaf explants of mature sandalwood (Santalum album L.) trees under in vitro conditions, In Vitro Cellular and Developmental Biology-Plant, 49(2): 216.

Singh M., Sonkusale S., Niratker Ch. and Shukla P. (2014). Micropropagation of Shorea robusta: an economically important woody plant, J. Forest Science, 60(2): 70-74.

Tilkat E., Onay A., Yildirim H. and Ayaz E. (2009). Direct plant regneration from mature leaf explants of pistachio, Pistacia vera L., Scientia Hort, 121(3): 361-365.

Webster S., Mitchell S.A. and Ahmad M.H. (2003). A novel surface sterilization method for reducing fungal and bacterial contamination of field grown medicinal explants intended for in vitro culture. Proceedings: Science and Technology for Economic Development: Technology Driven Agriculture and Agro-Processing, SRC,Jamaica.http://www.kitchenculturekit.com/surfaceSterilizationMitchell2003.pdf.