Differential Responses in Gas Exchange Variability, Water use Efficiency and Chlorophyll content in <i>Eucalyptus</i> Clones under Drought Stress

Romeet Saha; Kirti Chamling Rai; Anugrah Tripathi; Haripriya Kavidayal; Manoj Raturi; Priya Kumari; Charul Kainthola; Chander Shekhar; Santan Barthwal

doi:10.36808/if/2019/v145i5/145699

Differential Responses in Gas Exchange Variability, Water use Efficiency and Chlorophyll content in Eucalyptus Clones under Drought Stress

Authors

Romeet Saha Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand
Kirti Chamling Rai Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand
Anugrah Tripathi Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand
Haripriya Kavidayal Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand
Manoj Raturi Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand
Priya Kumari Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand
Charul Kainthola Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand
Chander Shekhar Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand
Santan Barthwal Forest Research Institute, P.O. New Forest, Dehradun, Uttarakhand

DOI:

https://doi.org/10.36808/if/2019/v145i5/145699

Keywords:

Eucalyptus, Drought, Water Use Efficiency, Photosynthesis, Chlorophyll Content, Gas Exchange, Stomatal Density, Stomatal Size.

Abstract

Eight different clones of Eucalyptus were screened for their gas exchange variability, water use efficiency, stomatal parameters and chlorophyll content. Drought stress was imposed thirty days after transplanting wherein irrigation was completely stopped while control plants were irrigated daily throughout the period of the experiment. Measurements were taken in both stressed and control plants and all the clones showed a decrease in gas exchange variability and a positive increase in water use efficiency. Stomatal parameters showed great variability between the clones but a positive correlation was established between water use efficiency and stomatal density. Among the different clones, clone 23 was found to be relatively drought tolerant in terms of water use efficiency.

References

Boyer J.S., Wong S.C., Farquhar C.D. (1997). CO , and 2 water vapor exchange across leaf cuticle (epidermis) at various water potentials. Plant Physiology 114: 185-191. Cambridge.

Chakir S., Jensen M., and Feige G. (1999). Osmotic and Atmospheric Dehydration Effects in the Lichens Hypogymnia Physodes, Lobaria Pulmonaria, and Peltigera Aphthosa: An in vivo Study of the Chlorophyll Fluorescence Induction. Photosynthetica, 37:393. https://doi.org/10.1023/A:1007151625527

Chaves M.M. (1991). Effects of water deficits on carbon assimilation. Journal of Experimental Botany 42: 1-16.

Cornic G. and Lawlor D.W. (2002). Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant, Cell and Environment, 25: 275-294.

Dhiman R. and Gandhi J. (2014) Status of Eucalypt Clonal Culture in India. ENVIS. Eucalypts in India. 60-116pp.

Farquhar G.D., Ehleringer J.R., and Hubick K.T. (1989). Carbon Isotope Discrimination and Photosynthesis. Annual Review of Plant Physiology and Plant Molecular Biology, 40(1): 503-537.

Gazanchian A., Hajheidari M., Sima N.K. and Salekdeh G.H. (2007). Proteome response of Elymuselongatum to severe water stress and recovery. J. Experimental Botany, 58: 291-300.

Ginwal H.S. (2014) Eucalypt Improvement: Efforts and Achievements in India. ENVIS. Eucalypts in India. 117-138pp.

Hiscox J.D. and Israelstam G.F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian J. Botany, 57(12): 1332-1334

Jaleel C.A., Ragpathi G., Beemarao S., Muthiah G. and Panneerselvam R. (2008). Differential responses in water use efficiency in two varieties of Catharanthus roseus under drought stress, C. R. Biologies 331.

Jones H.G. (1983). Plants and microclimate. Cambridge University Press Kawamitsu Y., Driscoll T. and Boyer J.S. (2000). Photosynthesis during dessication in an Intertidal Alga and a Land Plant. Plant Cell Physiol., 41: 344-353.

Kumar A., Shrivastava P. and Kumar R. (2017). Development and released of high yielding clones of Eucalyptus tereticornis Sm, (December).

Lal P., Dogra A.S., Sharma S.C and Chahal G.B.S. (2006). Evaluation of Different Clones of Eucalyptus in Punjab. Indian Forester, 132(11):1383-1390.

Liu S., Liu J., Cao J., Bai C. and Shi R. (2006). Stomatal distribution and character analysis of leaf epidermis of jujube under drought stress. J. Anhui Agricultural Science 34: 1315-1318.

Loustau A., Bosc A., Colin A., OgÃ©e J., Davi H., FranÃ§ois C., Dulrene E., DÃ©quÃ© M., Cloppet E., Arronays D., Le Bas C., Saby N., Pignard G., Hamza N., Granier A., BrÃ©da N., Ciais P., Viovy N. and Delage F. (2005). Modeling climate change effects on the potential production of French plains forests at the sub-regional level. Tree Physiology, 25: 813-823.

Mafakheri A., Siosemardeh A., Baharamnejad B., Struik P.C. and Sohrabi Y. (2010). Effect of drought stress on yield, proline and chlorophyll content in three chickpea cultivars. Australian J. Crop Science, ISSN 1835-2693 4: 580-585.

Manivannan P., Jaleel C.A., Kishorekumar A., Sankar B., Somasundaram R., Sridharan R. and Panneerselvam R. (2007), Changes in antioxidant metabolism of Vignaunguiculata (L.) Walp. by propiconazole under water deficit stress, Colloids Surf. B: Biointerfaces, 57: 69-74.

MartÃnez J.P. Ledent J.F, Bajji M., Kinet J.M. and Lutts S. (2003). Effect of water stress on growth, Na⁺ and K⁺ accumulation and water use efficiency in relation to osmotic adjustment in two populations of Atriplexhalimus L. Plant Growth Regulation, 41:63-73.

Martinez J.P., Silva H., Ledent J.F. and Pinto M. (2007). Effect of drought stress on the osmotic adjustment, cell wall elasticity and cell volume of six cultivars of common beans (Phaseolus vulgaris L.). European J. Agronomy, 26: 30-38.

Pohjonen V., Pukkala T., and Dechasa J. (1989). Growth of eucalypts in the Ethiopian highlands. Observations on semi permanent sample plots. Forestry Research Center, Ministry of Agriculture, Ethiopia, Res. Note, 3.

Shimazaki K.I., Doy M., Assmann S.M. and Kinoshita T. (2007). Light regulation of stomatal movement. Annual Review of Plant Biology, 58: 219-247.

Smirnoff N. (1995). Antioxidant Systems and Plant Response to the Environment. In: Environment and Plant Metabolism: Flexibility and Acclimation (Smirnoff, V., Ed.). BIOS Scientific Publishers, Oxford, 217-243.

Spence R.D., Wu H., Sharpe P.J.H. and Clark K.G. (1986). Water stress effects on guard cell anatomy and the mechanical advantage of the epidermal cells. Plant, Cell and Environment, 9: 197-202.

Yang L., Han M., Zhou G. and Li J. (2007). The changes of water-use efficiency and stoma density of Leymuschinensis along Northeast China Transect. Acta Ecologica Sinica, 27: 16-24.

Yordanov I., Velikova V. and Tsonev T. (2003). Plant responses to drought and stress tolerance. Bulg. J. Plant Physiol., Special Issue: 187-206.