Studies on Germination Behaviour of Seeds Harvested from Moringa Leaf Extract Sprayed Rauvolfia serpentina Plants
DOI:
https://doi.org/10.36808/if/2024/v150i8/170262Keywords:
Moringa Leaf Extract, Natural Biostimulant, R. serpentina, Seed Germination.Abstract
Natural biostimulants hold lot of potential in organic cultivation of medicinal plants, especially in those medicinal plants showing low to moderate seed germination. Rauvolfia serpentina is an economically important medicinal crop due to its root commercial value. However, stony coat and low seed quality imposes low to moderate seed germination in this plant. An organic approach is very essential to improve the plant health which leads to the production of good healthy seeds with high germination percentage. Moringa oleifera leaf extract (MLE) has been widely used as natural biostimulant for the improvement of crop growth and productivity. Thus, the present study was undertaken to assess the germination behaviour of harvested seeds from MLE sprayed R. serpentina plants. The field experiment was established under Randomized Block Design and plants were foliar sprayed with different doses of aqueous MLE i.e. 3.0%-21.0% for one year. Mature seeds were harvested and subjected to germination test to record germination percentage (G%), mean germination rate (MGR), mean germination time (MGT) and germination index (GI). The study revealed that seeds collected from 12% MLE sprayed R. serpentina plants have significant higher germination percentage (80), MGT (8.72), MGR (0.117), and GI (6.90) followed by 9% of MLE sprayed plants as compared to control. Thus, the aqueous 9-12% MLE foliar doses showed potential as natural biostimulant for production of healthy R. serpentina seed with better germination percentage.References
Argueso C.T, Ferreira F.J. and Kieber J.J. (2009). Environmental perception avenues: the interaction of cytokinin and environmental response pathways, Plant, Cell and Environment, 32: 1147-1160.
Arif Y., Bajguz A. and Hayat S. (2023). Moringa oleifera Extract as a Natural Plant Biostimulant. Journal of Plant Growth Regulation, 42: 1291–1306.
Basra S.M.A., Farooq M., Tabassam R. and Ahmad N. (2005).Physiological and biochemical aspects of pre-sowing seed treatments in fine rice (Oryza sativa L.), Seed Science and Technology, 33: 623–628.
Bindu S., Rameshkumar K.B., Kumar B., Singh A. and Anilkumar C. (2014). Distribution of reserpine in Rauvolfia species from India – HPTLC and LC–MS studies, Industrial Crops and Products, 62: 430–436.
Dorkar B.K. (2021). Medicinal properties and seed germination study in Rauvolfia serpentina (L.) Benth.exKurz (Sarpagandha), International Journal of Advanced Research in Science, Engineering and Technology, 8: 8418.
Ghate V.U., Gajendragadkar M.P. and Jadhav A.B. (2019). Review of Rauvolfia serpentina (L.) Benth. Ex Kurz, International Journal of Research, 8(6): 414–421.
Goraya G.S. and Ved D.K. (2017). Medicinal Plants in India: An Assessment of their Demandand Supply. National Medicinal Plants Board, Ministry of AYUSH, Government of India, NewDelhi and Indian Council of Forestry Research & Education, Dehradun, pp. 27.
Hassan F.A.S. and Fetouh M.I. (2019). Does a novel preservative of Moringa leaf extract improve the longevity and postharvest quality of gladiolus cut spikes? Scientia Horticulturae, 250: 287-293.
Indian Council of Medical Research. (2010). Rauvolfia serpentine (L) Benth. exKurz, Quality Standards of Indian Medicinal Plants, A publication of Medicinal Plant Unit, Indian Council of Medical Research, New Delhi, pp.272-282.
Jain V., Singh D. and Saraf S. (2003). In-vitro micro-propagation of Rauvolfia serpentina through multiple shoot generation, Ancient Science of Life, 23: 1-5.
Jardin P. (2015). Plant biostimulants: Definition, concept, main categories and regulation, Scientia Horticulturae, 196: 3-14.
Khobragade P. and Khobragade M. (2017). Propagation of Rauvolfia serpentina (L) Benth.ex Kurz – a small approach towards creating awareness for its conservation and propagation, International Journal of Pharmaceutical Sciences and Research, 8: 70-73.
Kim J.G., Takami Y., Mizugami T., Beppu K., Fukuda T. and Kataoka I. (2006). CPPU application on size and quality of hardy kiwifruit, Scientia Horticulturae, 110: 219-222.
Kirtikar K.R. and Basu B.D. (1993). Indian Medicinal Plants. 2nd ed. Calcutta: Dehra Dun Publishers. 11: pp 1550-1551.
Kumar, S., Kumari D., Singh B. (2022). Genus Rauvolfia: A review of its ethnopharmacology, phytochemistry, quality control/quality assurance, pharmacological activities and clinical evidence, Journal of Ethnopharmacology, 295: 115327
Li J., Van Gerrewey T. and Geelen D. (2022). AMeta-Analysis of Biostimulant Yield Effectiveness in Field Trials, Frontiers in Plant Science, 13: 836702.
Mishra S.P., Singh P., Singh S., Das R. and Prasad R.S. (2013). Moringa oleifera leaf extract as biostimulant for increasing pea yield, Indian Forester, 139: 562-563.
Mukherjee E., Gantait S., Kundu S., Sarkar S. and Bhattacharyya S. (2019). Biotechnological interventions in the genus Rauvolfia: recent trends and imminent prospects, Applied Microbiology and Biotechnology, 103: 7325-7354.
Mukherjee E., Sarkar S., Bhattacharyya S. and Gantait S. (2020). Ameliorated reserpine production via in vitro direct and indirect regeneration system in Rauvolfia serpentina (L.) Benth.exKurz, ABiotech, 10: 294.
Norrie J. and Keathley J.P. (2006). Benefits of Ascophyllum nodosum marine-plant extract applications to 'Thompson seedless' grape production, Acta Horticulturae, 727: 243–247.
Pandey A.K. and Mandal A.K. (2010). Influence of propagation techniques and harvesting time on root yield and alkaloid contents of Rauvolfia serpentina, Journal of Natural Remedies, 10: 44-49.
Paul D., Paul N.K. and Basu P. (2008). Seed germination response of Rauvolfia serpentina (L.) Benth. Ex Kurz to certain physical and chemical treatments, Journal of Biosciences, 16: 129-131.
Pawar G.S. and Guru S.K. (2012). Influence of Gibberellic acid and seed coat removal on the seed germination behaviour of Rauwolfia serpentina (L.) Benth.ex Kurz under controlled environment, Journal of Non-Timber Forest Product, 19: 5-8.
Phiri C. (2010). Influence of Moringa oleifera leaf extracts on germination and early seedling development of major cereals, Agriculture and Biology Journal of North America, 1: 774-777.
Sayal N. and Thakur A. (2018). Effect of dormancy breaking treatments on germination, vigour and physio-biochemical changes in seeds of Rauvolfia serpentina (L.) Benth.ex Kurz endangered medicinal species, The Journal of Indian Botanical Society, 97: 52-58.
Singh P., Singh S. and Reddy G.R.S. (2020). Assessment of Moringa oleifera Lam. leaf extract as natural biostimulant on tomato (Lycopersicon esculentum Mill.) crop production, Journal of Tree Science, 39: 16-23.
Sharma A., Devkota D., Thapa S., Sapkota M. and Bista B. (2021). Improving Germination and Stand Establishment of Kiwifruit (Actinidia deliciosa Cv. Hayward) Seed Through Media Selection and Hormonal Use in Dolakha, Nepal, Tropical Agrobiodiversity, 2(1): 16-21.
Soni R., Jaiswal S., Bara J.K. and Saksena P. (2016). The Use of Rauwolfia serpentina in Hypertensive Patients, Journal of Biotechnology and Biochemistry, 2(5): 28-32.
Tariyal Y. S., Ansari S. and Prasad P. (2021). Organic Cultivation of Medicinal and Aromatic Plants: AReview, Journal of Stress Physiology and Biochemistry, 17(1): 129-133.
Tiwari A.K., Verma P.P.S., Kumar D., Singh S., Kumar R., Padalia R.C. and Upadhyay R.K. (2021). Germination Behaviour of Rauvolfia serpentina (L.) seeds with the use of different Seed Treatments, Indian J. Agricultural Research, 1-5.
Tripathi H., Suresh R., Kumar S. and Khan F. (2017). International trade in medicinal and aromatics plants: A case study of past 18 years, Journal of Medicinal and Aromatic Plant Sciences, 39: 1-17.
Ved D.K. and Goraya G.S. (2007). Demand and Supply of Medicinal Plants in India. NMPB, New Delhi and FRLHT, Bangalore, India, pp 18.
Yakhin I.O., Labyanov A.A., Yakhin A.I. and Brown H.P. (2017). Biostimulant in Plant Sciences: A Global Perspective, Frontiers in Plant Science, 7: 2049.
Yaseen A. and Hájos M.T. (2020). Study on Moringa tree (Moringa oleifera Lam.) leaf extract in organic vegetable production: Areview, Research on Crops, 21: 402-414.
Yasmeen A., Basra S.M.A., Rashid A. and Wahid A. (2011). Performance of late sown wheat in response to foliar application of Moringa oleifera L. leaf extract, Chilean Journal of Agricultural Research, 72: 92-97.
Zabadal T.J. and Bukovac M.J. (2006). Effect of CPPU on fruit development of selected seedless and seeded grape cultivars, Hort Science, 41: 154-157.
Zulfqar F., Casadesús A., Brockman H. and Munné-Bosch S. (2020). An overview of plant-based natural biostimulants for sustainable horticulture with a particular focus on Moringa leaf extracts. Plant Science, 295: 110194.
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