Rapid Regeneration of <I>Bambusa nutans</I> Munro. from Shoot and Leaf Explants

Abstract

Callus induction was achieved on pre-injured explants. Performance of explants derived from in-vitro multiplying cultures was better than those from mature plants. 2, 4-D proved to be superior in inducing callogenic response than NAA or IBA. Enhancement of callogenic efficiency of in-vitro derived explants was observed when BAP (2.5μM) was supplemented to auxin-enriched medium. Induced callus was multiplied on multiplication medium (5μM 2,4-D+ 1.25μM BAP) for varying periods but optimal multiplication rate for shoot callus (1.69) and leaf callus (1.39) was obtained after subculture cycle of three weeks. Shoot and bud regeneration efficiency of calli induced from leaves and shoot varied as well as their phytohormonal requirements. Buds and shoots were regenerated at higher frequency on shoot calli cultured on multiplication medium supplemented with (5μM BAP+ 1.25μM NAA) whereas for leaf calli it was 7.5μM BAP + 1.25μM NAA. Spontaneous root regeneration occurred at frequency of 61% on shoots regenerated on multiplication medium.