Standardization of Buffers and Substrate Solutions for <I>in-vivo</I> Nitrate Reductase Activity in <I>Cinnamomum camphora</I> (L.) J.Presl Leaves

Meenu Singh Sengar; S. P. Chaukiyal; Priyanshu Sharma

doi:10.36808/if/2016/v142i11/105611

Standardization of Buffers and Substrate Solutions for in-vivo Nitrate Reductase Activity in Cinnamomum camphora (L.) J.Presl Leaves

Authors

Meenu Singh Sengar Gautama Budha University, Greater Noida Uttar Pradesh
S. P. Chaukiyal Plant Physiology Discipline, Botany Division, Forest Research Institute, Dehradun (Uttarakhand)
Priyanshu Sharma Gautama Budha University, Greater Noida Uttar Pradesh

DOI:

https://doi.org/10.36808/if/2016/v142i11/105611

Keywords:

Buffer, Cinnamomum camphora, Nitrate Reductase Activity, Substrate.

Abstract

The assessment of in-vivo nitrate reductase (NR) activity in leaves of Cinnamomum camphora was performed to obtain the most suitable substrate and phosphate buffer solutions for the estimation of maximum in-vivo NR activity. Phosphate buffer (0.1M and 0.2M) solution with pH ranging from 7.0 to 7.8 and concentration of substrate (KNO₃) solution varying from 0.05, 0.10, 0.15, 0.20 and 0.25M were used in the present experiment. Highest nitrate reductase activity was recorded in the combination of phosphate buffer 0.2M solution having pH 7.2 and substrate solution of the concentration 0.15M.

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