In Vitro Propagation of <I>Elaeocarpus Sphaericus</I>

J. M. S. Chauhan; Prabha Bisht; Monika Panwar; Ajay Thakur

doi:10.36808/if/2015/v141i2/60663

In Vitro Propagation of Elaeocarpus Sphaericus

Authors

J. M. S. Chauhan Tissue Culture Discipline, Genetics and Tree Propagation Division, Forest Research Institute, Dehradun (Uttarakhand)
Prabha Bisht Tissue Culture Discipline, Genetics and Tree Propagation Division, Forest Research Institute, Dehradun (Uttarakhand)
Monika Panwar Tissue Culture Discipline, Genetics and Tree Propagation Division, Forest Research Institute, Dehradun (Uttarakhand)
Ajay Thakur Tissue Culture Discipline, Genetics and Tree Propagation Division, Forest Research Institute, Dehradun (Uttarakhand)

DOI:

https://doi.org/10.36808/if/2015/v141i2/60663

Keywords:

Elaeocarpus Sphaericus, In-Vitro Propagation, Nodal Explant, Axillary Shoot Sprouting.

Abstract

The present study involves in-vitro clonal propagation of Elaeocarpus sphaericus through axillary bud sprouting and subsequent shoot regeneration. Optimal proliferations of axillary shoot were induced on MS medium supplemented with BAP (1.5 mg/l) and KN (1.5 mg/l). Optimal shoot multiplication was induced on MS medium fortified with BAP (1.5mg/l), KN (1.5 mg/l) and CH (100 mg/l). MS medium supplemented with 1.0 mg/l BAP and 50 mg/l ADS was used for routine sub-culturing of multiple shoots at periodic interval of every 3 weeks. Of the 6 treatments (3 each of IBA and NAA) imposed for rooting of shoots, 1/2 MS + 1.0 mg/l IBA induced 80% rooting. Plants were hardened in vitro and acclimatized in hardening chamber and subsequently transferred to field.

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