Tissue culture (Micropropagation) of <I>Dalbergia sissoo</I> Roxb. as affected by Genotypic configuration

Meena Bakshi; Aradhna Chauhan; Ankur Vichitra

doi:10.36808/if/2018/v144i9/132678

Tissue culture (Micropropagation) of Dalbergia sissoo Roxb. as affected by Genotypic configuration

Authors

Meena Bakshi Forest Research Institute, Dehradun (U.K)
Aradhna Chauhan Sai Institute of Paramedical and Allied Sciences, Rajpur Road, Dehradun
Ankur Vichitra Forest Research Institute, Dehradun (U.K)

DOI:

https://doi.org/10.36808/if/2018/v144i9/132678

Keywords:

Dalbergia sissoo, Genotypic Variability, In-vitro, Nodal Explants, MS Medium.

Abstract

Genotypic variation in in-vitro performance of 5clones of Dalbergia sissoo revealed significant variations in bud proliferation and multiplication. The survival percentage of explants inoculated on MS medium with 1.0 mg/l BAP+ 0.1 mg/l NAA ranged from 56.9 to 79.1 % with the maximum being in C14 (Haridwar). Maximum bud break (69.4%) was found in C 114 (Nepal) while number of buds was maximum (2.79) in C86. The shoot number and length were maximum (1.65 and 2.05cm respectively) in C14. Subculturing of microshoots on 1.0 mg/l BAP+ 0.25 mg/l NAA revealed maximum multiplication rate of 4.02 in C14 with maximum shoot number (3.07) while shoot length was maximum (2.43 cm) in C 86. The maximum rooting (40.5%) with 1mg/l IBA was observed in C86 (Rajasthan) at par with C14 (40%). Significantly (0.1%) maximum number of roots (2.46) and root length (2.63 cm) were also recorded in C14 with high survival rate. Among 5 selected clones, C14 (Haridwar) showed best performance in shooting as well as rooting in vitro, hence can be multiplied reliably to be put under agro-forestry plantations.

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