Molecular Identification and Phylogenetics Studies of Erythrina arborescens Roxb.
DOI:
https://doi.org/10.36808/if/2020/v146i1/141638Keywords:
Erythrina arborescens, RbcL Gene, Molecular Identification, Phylogeny, Evolutionary Linkages.Abstract
Erythrina arborescens belonging to the family Leguminosae (Subfamily: Fabaceae) is an important broad leaved tree species used for medicinal and ornamental purpose in lower Himalayan region. Due to excessive lopping by local communities, its population status is declining at alarming rate thus germplasm conservation is urgently needed. Ambiguity in morphology led to create trouble in identification with other Erythrina species. DNA was extracted from leaf samples collected from taxonomically identified source of E. arborescens. PCR amplicons were subjected to sequence analysis based on rbcL gene. Neighbor-joining method was used to construct phylogenetic tree with sequence of query species and identical sequences retrieved from NCBI GenBank database through BLAST. No sequence for this species was identified from GenBank databases for this plant. The rbcL gene sequence of E. arborescens was the first record to molecular characterization and could be used for the identification of this species at molecular level reported from any part of the world through BLAST analysis if the reference domain for the molecular identical sequences is submitted to GenBank in future. Query sequence of E. arborescens showed 100% identity with the rbcL gene sequence of Erythrina sigmoidea already submitted to the NCBI GenBank database. Erythrina arborescens is closely related with Erythrina flabelliformis based on nucleotide homology and phylogenetic analysis.References
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