Pheno-Morphological and Biochemical Characterization of <i>Rhizobium</i> sp Associated with Khejri Seedlings Isolated from Different Geographical Location of Rajasthan

Sangeeta Singh; Tanmaya Kumar Bhoi; Sunil Choudhary; Vipula Vyas; Kuldeep Sharma

doi:10.36808/if/2023/v149i7/169455

Pheno-Morphological and Biochemical Characterization of Rhizobium sp Associated with Khejri Seedlings Isolated from Different Geographical Location of Rajasthan

Authors

Sangeeta Singh Forest Protection Division, ICFRE- Arid Forest Research Institute, Jodhpur, Rajasthan, 342005
Tanmaya Kumar Bhoi Forest Protection Division, ICFRE- Arid Forest Research Institute, Jodhpur, Rajasthan, 342005
Sunil Choudhary S.B.R.M. Government College, Nagaur, Rajasthan, 341001
Vipula Vyas Forest Protection Division, ICFRE- Arid Forest Research Institute, Jodhpur, Rajasthan, 342005
Kuldeep Sharma Forest Protection Division, ICFRE- Arid Forest Research Institute, Jodhpur, Rajasthan, 342005

DOI:

https://doi.org/10.36808/if/2023/v149i7/169455

Keywords:

Characterization, Prosopis cineraria, Rhizobium.

Abstract

This study aimed to isolate, characterize and purify microbial strains of Rhizobium specific to Khejri in a bid to increase this legumeâ€™s productivity. In the present study 24 bacterial isolates were isolated from root nodule of Prosopis cineraria L. from 5 different districts of Western Rajasthan. The isolates were investigated for their morphological, conformity, phenotypic and biochemical characterisation. Most of the isolates showed similar colony morphology and produced white or creamy white and raised colonies when grown on YEMA plates and theys were rod shaped gram negative. Phenotypic characterization of 24 isolates confirmed that most of the strains tested for BTB reaction were acid producers. Six isolates were showing NaCl tolerance up to 3% (w/v). Most of the tested isolates were able to tolerate a wide range of pH (5 to 11). Moreover, in biochemical characterisation most of the isolates showed positive result for catalase, phosphate solubilization, nitrate reductase and oxidase test and negative result for ammonia production and chitinase test. Furthermore, in the nursery experiment it was found that all the inoculated seedlings showed significant differences in terms of number of root nodules, collar diameter, root and shoot length, root and shoot (wet and dry) weight as compared to the uninoculated control plants. Among the 24 Rhizobium isolates PCC, PCN and PCsk were found superior than other isolates in terms of number of nodule and plant dry matter and has been selected for a future field trial.

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