Genetic Diversity of Bamboo Species in Andaman and Nicobar Islands Revealed through Dna Markers and Morphological Traits
DOI:
https://doi.org/10.36808/if/2017/v143i5/114954Keywords:
Bamboo, ISSR, RAPD, Morphological Traits, Genetic Diversity, Association.Abstract
Analysis started by using forty eight markers (Twenty four each of ISSR and RAPD) that allowed us to distinguish 10 bamboo species. Out of forty eight, twelve primers were used for identification and for establishing a profiling system to estimate genetic diversity. A total of six hundred and fourteen alleles were amplified out of this hundred and thirty seven distinct polymorphic DNA fragments, ranging from 1.15 - 0.20 kb were amplified by 12 selected primers. The morphological observations recorded at field level viz., average height of mature culms, average inter node distance and average diameter of the culm. It was observed that Bambusa bambos and Bambusa tulda were very similar to each other for all three characters. While Schizostachyum kalpongianum showed very different type of characters in all three traits. The genetic similarity analysis was conducted based on presence or absence of bands, which revealed a wide range of variability among the species. Combined cluster analysis revealed that the ISSR and RAPD markers studied were highly efficient in differentiating the bamboo species up to genera level. Upon endemic species studied, it is observed that two genus Gigantochloa and Schizostachyum were grouped to separate cluster whereas Dendrocalamus calostachys were grouped with Dendrocalamus strictus. Thus, the morphological characterization was confirmed by the genetic diversity analysis.References
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