<I>In-vitro</I> Callus Development for Organogenesis in Lac Host Plants

Abstract

Besides traditional propagation and breeding practices modernized methods of plant tissue culture is considered as a crucial factor for lac host plant improvement programme. Therefore, under the current study plant parts like seed cotyledons and axillary buds of lac host plants were cultured under different auxin (2,4-D) concentrations to find their ability to produce callus. Mostly in all cases,2,4-D concentrations of 4-10 ppm resulted in maximum callus development. The results open up the possibility of improvement in lac host plant through biotechnology. However, further studies for organogenesis and regeneration into complete plantlets are required with utmost importance.